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Qualitative and quantitative tests of antimicrobial properties
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Jul . 30, 2024 17:33 Torna à a lista

Qualitative and quantitative tests of antimicrobial properties


Commonly used, representative test standards

Test principle

The antibacteria

l sample is placed close to the AGAR plate surface inoculated with a certain amount of specific microorganisms, and after a period of contact culture, it is observed whether there is a bacteriostatic zone around the sample and whether there is a microbial growth on the contact surface between the sample and the AGAR, so as to judge whether the sample has antibacterial properties.

Effect evaluation
Qualitative test is suitable for determining whether the product has antibacterial effect. When there is antibacterial zone around the sample or no bacterial growth on the contact surface between the sample and the medium, it indicates that the sample has antibacterial performance, but the antibacterial activity of the textile can not be judged by the size of the antibacterial zone. The size of the antibacterial zone can reflect the dissolution of the antibacterial agent used in the antibacterial product

 

Commonly used, representative test standards

Test principle

After quantitative inoculation of the test suspensions of the treated samples and the non-treated control samples, after a certain period of incubation, the antibacterial effect of the textiles was quantitatively evaluated by comparing the number of bacterial growth in the tested samples and the control samples. In quantitative detection methods, the commonly used methods are absorption method and oscillation method.

 

The absorption method is to absorb a certain amount of bacterial suspension in the control sample and the antibacterial test sample, culture it in the static state, and eluate the test bacteria on the sample after a certain period of time for detection and comparison, so as to evaluate its antibacterial performance. It is suitable for the antibacterial performance test of antibacterial fabrics with certain water absorption.

The oscillation method is to completely soak the control sample and the antibacterial test sample in the buffer containing a certain concentration range of the test bacterial solution, continuous oscillation culture, after a certain period of time to detect and compare the bacterial solution, which is suitable for the antibacterial performance test of non-dissolved antibacterial textiles and samples with poor water absorption.

 

Effect evaluation

The quantitative test method reflects the antibacterial activity of antibacterial textiles in the form of a percentage or value of antibacterial rate or antibacterial value, and the higher the antibacterial rate and antibacterial value, the better the antibacterial effect. Some test standards give the corresponding evaluation basis.

Now do you know how to choose the antibacterial test method? Consider the following three points:

Test requirement

Just want to know if there is an antibacterial effect - choose a qualitative test

Want to know if the antibacterial effect is good - choose a quantitative test

Rating required - Select criteria that can be rated (e.g., above)

Product characteristics

Non-soluble antibacterial textiles and samples with poor water absorption are preferred by the oscillation method.

Product use or enterprise standard requirements

For example, if a product claims to have an antibacterial effect on a strain other than the standard, testing for that strain needs to be increased.

 

Warm reminder

The test of antibacterial functional textiles not only has antibacterial performance, but also has the requirements of safety performance, and the dissolution test is one of the indexes of its safety performance. Most of our antibacterial textiles contact the human body, they should first be safe, and then antibacterial effective, we do not ignore the dissolution of this important indicator oh!

 

 

 

 

 

 

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